propose 2 strategies for drug enzyme inhibition

Research Proposal (Outcome Assessment)

Write out in full of all aspects of your outline for one potential drug target. You must make sure that the motivation and implication of the experiments are explained. You must propose at least two strategies for the design of your inhibitor (may include mechanism-based, transition state analogs, substrate analogs, computational designed, etc). You must include figures demonstrating your drug design strategies. You must discuss the mechanism of the reaction. You must include the relevant reaction pathway that your enzyme involved in. You must include all the relevant references.

I. Abstract

Motivation/problem statement
Hypothetical outcomes
Implication of experiments

II. Introduction

Background on the topics
Pathways (metabolic or regulatory) was involved. Use figures
Protein Structural information, active site interactions use figures

III. Drug Design Methods

At least 2 strategies used (equal emphasis to both strategies)
List the two strategies
Two strategies clearly explained
At least four inhibitors are proposed
Rationale for design method clearly explained
Interactions shown between drug and target
Evolution of proposed drugs proposed

IV. Proposed experiments/hypothetical results and outcomes

Present all potential results of experiments
Do not present so many hypothetical outcomes that you lose the sight of the main problem statement
Give visual models which clearly describe your arguments

V. Discussion of hypothetical outcomes
VI. Conclusion
VII. References


Look for a review article that describes the genetic basis of disease.
Look for a paper where they have recently solved crystal structure with substrate or product, where the enzyme is involved in disease function

Look for a paper that discussed inhibitors of one enzyme in a pathway, maybe inhibiting the enzyme before or after that step has not explored yet.

How to Review the Paper

Read the paper
Include comments, corrections, and suggestions or write down the questions raised by reading the paper throughout, where relevant.
Rate the paper by following criterion (C = not at all, B = somewhat, A = very well).